Eukaryotic genome instability in light of asymmetric DNA replication
نویسندگان
چکیده
منابع مشابه
DNA replication stress, genome instability and aging
Genome instability is a fundamentally important component of aging in all eukaryotes. How age-related genome instability occurs remains unclear. The free radical theory of aging posits oxidative damage to DNA and other cellular constituents as a primary determinant of aging. More recent versions of this theory predict that mitochondria are a major source of reactive oxygen species (ROS) that ca...
متن کاملGenome-wide model for the normal eukaryotic DNA replication fork.
To investigate DNA replication enzymology across the nuclear genome of budding yeast, deep sequencing was used to establish the pattern of uncorrected replication errors generated by an asymmetric mutator variant of DNA polymerase δ (Pol δ). Sequencing of 16 genomes identified 1,206-bp substitutions generated over 33 generations by L612M Pol δ in a mismatch repair defective strain. Alignment of...
متن کاملDNA replication in eukaryotic cells.
The maintenance of the eukaryotic genome requires precisely coordinated replication of the entire genome each time a cell divides. To achieve this coordination, eukaryotic cells use an ordered series of steps to form several key protein assemblies at origins of replication. Recent studies have identified many of the protein components of these complexes and the time during the cell cycle they a...
متن کاملSURVEY AND SUMMARY DNA replication stress, genome instability and aging
Genome instability is a fundamentally important component of aging in all eukaryotes. How agerelated genome instability occurs remains unclear. The free radical theory of aging posits oxidative damage to DNA and other cellular constituents as a primary determinant of aging. More recent versions of this theory predict that mitochondria are a major source of reactive oxygen species (ROS) that cau...
متن کاملThe DNA replication fork in eukaryotic cells.
Replication of the two template strands at eukaryotic cell DNA replication forks is a highly coordinated process that ensures accurate and efficient genome duplication. Biochemical studies, principally of plasmid DNAs containing the Simian Virus 40 origin of DNA replication, and yeast genetic studies have uncovered the fundamental mechanisms of replication fork progression. At least two differe...
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ژورنال
عنوان ژورنال: Critical Reviews in Biochemistry and Molecular Biology
سال: 2015
ISSN: 1040-9238,1549-7798
DOI: 10.3109/10409238.2015.1117055